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2x TaqMan Quantitative Real-Time PCR Master Mix, TaqMan qPCR Mix

$95.00 $60.00

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TaqMan Quantitative Real-Time PCR Master Mix, 2x TaqMan qPCR Master Mix with ROX Dye. 200 Reactions

Our TaqMan Quantitative Real-Time PCR Master Mix — TQ2110 ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers, TaqMan probes and templates. The master mix includes a 5’ to 3’ exonuclease activity to cleave TaqMan probes that hybridize to target sequences, releasing fluorophore during probe displacement. With TaqMan probes, the master mix features high specificity and high sensitivity (Fig. 1). The ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX) contains hot-start Taq polymerase in an optimized buffer that allows for sensitive and precise amplification, real-time tracking of the amplification process, and simultaneous quantification for targeted DNA molecules. The master mix includes ROX reference dye for the normalization of each qPCR assay. The ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process.

Features
• High sensitivity
• High specificity
• With ROX reference dye

Storage
Aliquot to avoid multiple freeze-thaw cycles.
Protect from light.
-20°C ≥ 12 months

Application
Quantitative real-time PCR
Quantitative two-step real-time PCR

Guide For Use
For best results of qPCR by using 2X Q-PCR Master Mix (TaqMan, ROX), the following procedures and components listed in Tables 1 to 3 are strongly suggested.

Table 1. Recommended reaction mixture set up for TaqMan real-time qPCR

Reaction Components

Volume

Final Concentration

Template

2ul

CDNA: 76 fg ~ 20 ng

gDNA: 4 ng ~ 20 ng

Plasmid: 103 ~ 108 molecules

Forward Primer*

varied

125 ~ 900 nM

Reverse Primer*

varied

125 ~ 900 nM

TaqMan Probe*

varied

100 ~ 200nM

2x qPCR Master Mix (TaqMan, ROX)

10ul

1x

ddH2O

Up to 20ul

Total Volume

20ul

* The PCR primer and probe concentration for an optimal qPCR reaction may vary according to the primer’s and probe’s properties and template conditions.

Table 2. Recommended real-time PCR program for: Two-step cycle

Steps

Temp

Time

Cycles

Template Denaturation and Enzyme Activation

95℃

10 min

1

Denaturation

95℃

15 sec

40

Annealing/Extension

60℃

60 sec

Table 3. Recommended real-time PCR program for: Three-step cycle

Steps

Temp

Time

Cycles

Template Denaturation and Enzyme Activation

95℃

10 min

1

Denaturation

95℃

15 sec

40

Annealing

60℃

30 sec

Extension

72℃

30 sec

 

TaqMan-qPCR-Master-Mix

Fig. 1. The amplification plot of real-time PCR with cDNA templates ranging from 76 fg to 20 ng in quantity, analyzed by using TQ2110 ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX) for qPCR amplification.

Thermo-scientific-TaqMan-Master-Mix

Fig. 2. SMOBIO’s TQ1110 ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX) shows better fluorescent signal as compared to a similar product (Brand A).

Shipping cost
Please note this item is NOT participating the “Free Shipping on $200” rule. A $40 invoice will be sent for a standard 2nd-day shipping. If you order our other Taq PCR related products we only charge shipping cost once. Check our other Taq PCR related products. Buy more save more.

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