$85/pack of 0.5ml 3 color Prestained Protein Ladder Marker For Western Blotting, SDS-PAGE Gel Elecrophoresis (cat# PM001-0500). Free priority mail shipping on orders $200 and more.
With, one of our best sellers, 3 Tri-colored prestained protein marker, you can load up over 300 wells for Western Blotting application. Free priority mail shipping for customers in the 48 Continental United States AND Alaska, Hawaii, Puerto Rico, the Virgin Islands.
The BLUltra Prestained Protein Ladder is a three tri color protein standard with 10 pre-stained proteins covering a wide range of molecular weights from 6.5 to 270 kDa. Proteins are covalently coupled with a blue chromophore except for three reference bands (two orange bands at 30 kDa and 270 kDa and one green band at 52 kDa) when separated on SDS-PAGE (Tris-glycine buffer). The BLUltra Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in the gel loading buffer and is ready to use.
• 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
• 2.5 μl per well for general Western blotting transferring. So, basically you can load 200 pieces of SDS PAGE Gels.
62.5 mM Tris-H3PO4 (pH 7.5 at 25 °C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3, 33% glycerol.
Under suggested conditions, BLUltra Prestained Protein Ladder resolves 10 major bands in 4-12% Bis-Tris Gel(MES buffer) and after Western blotting to the nitrocellulose membrane.
Stable for up to 2 weeks at 25°C.
Stable for up to 3 months at 4°C.
For long term storage (24 months), store at -20°C.
Note. The apparent molecular weight (kDa) of each protein has been determined by calibration against unstained protein standards; supplemental data should be considered for more accurate adjustment in different electrophoresis conditions.
All products are for research use only.
Caution: Not intended for human or animal diagnostic or therapeutic uses.
We have another tri-color prestained protein marker PM2500 which has 10 bands from 10 to 180 kDa and a little bit less inexpensive.
The shipping cost for each case ordered is $6.50, but will be automatically waived at checkout on orders of $200 and more.