Our ExcelBand 3-Color Extra Range 3-Color Pre-Stained Protein Markers (Cat# PM2800) are ready-to-use three-color protein marker with 13 pre-stained proteins covering an extra range of molecular weights from 10 to 310 kDa in Tris-Glycine buffer (9 to 290 kDa in Bis-Tris (MOPS) buffer and 10 to 290 kDa in Bis-Tris (MES) buffer). Proteins in this extra range ExcelBand 3 color prestained protein marker are covalently coupled with a blue chromophore except for three reference bands (one green and two red bands at 25 kDa and 75, 310 kDa respectively) when separated on SDS-PAGE in Tris-Glycine buffer.
The PM2800 ExcelBand 3-color Extra Range Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (nitrocellulose, PVDF, or nylon) and for approximating the size of proteins. The marker is supplied in the gel loading buffer and is ready to use. Do NOT heat, dilute, or add reducing agents before loading.
Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25℃), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).
Under suggested conditions, PM2800 3-color Extra Range Protein Marker resolves 13 major bands in 12% SDS-PAGE (Tris-Glycine buffer) and after Western blotting to nitrocellulose membrane.
250ul/vial, 2 vials of 250ul. Total 500ul
4°C ≥ 3 months
-20°C ≥ 24 months
Migration pattern of PM2800 in different electrophoretic condition
The shipping cost for each order placed is $6.50, but will be automatically waived at checkout on orders of $200 and up.