SMOBIO 3-color PreStained Protein Ladder (Cat# PM5100) is High Range, ready-to-use three-color protein ladder with 14 pre-stained proteins covering a wide range of molecular weights from 10 kDa to 245 kDa in Tris-Glycine Buffer (9 to 235 kDa in Bis-Tris (MOPS) buffer and 10 to 235 kDa in Bis-Tris (MES) buffer). Proteins in this 3 color prestained protein marker are covalently coupled with different chromophores for easy identification of bands, with three reference proteins carrying enhanced intensity corresponding to a blue band at 20 kDa, green at 40 kDa, and red at 75 kDa, respectively, as separated on SDS-PAGE (Tris-Glycine buffer). The PM5100 3-color PreStained Protein Ladder High Range is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5), 2% SDS, 0.2 mM DTT, 3.6 M urea, and 15% (v/v) glycerol).
Under suggested conditions, PM5100 ExcelBand 3-color Pre-Stained Protein Ladder High Range resolves 14 major bands in 15% SDS-PAGE (Tris-Glycine buffer) and after Western blotting to nitrocellulose membrane.
250ul/vial, 2 vials of 250ul. Total 500ul
4°C ≥ 3 months
-20°C ≥ 24 months
The shipping cost for each order placed is $6.50, but will be automatically waived at checkout on orders of $200 and up.