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ExcelTaq Blood Direct Taq DNA Polymerase, Blood Direct PCR 2000 Reactions

$85.00 $60.00

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TP2000 ExcelTaq Blood Direct Taq DNA Polymerase, Blood Direct PCR, 5U/ul, 500U/Pack

The ExcelTaq Blood Direct Taq DNA polymerase (TP2000) is designed for amplifying target DNA directly from whole blood, eliminating the need for a lengthy DNA isolation process —Blood Direct PCR. The ExcelTaq Blood Direct Taq DNA polymerase is highly tolerant in the presence of PCR interfering/inhibiting substances in blood (Fig 1). The ExcelTaq Blood direct PCR Master Mix is ideal for high-throughput screening of blood samples for high reproducibility. The ExcelTaq Blood Direct DNA polymerase includes a pair of positive control primers (CCR5) that are compatible with primate blood samples.

Blood-Direct-PCR

Fig. 1. The ExcelTaq Blood Direct DNA Polymerase amplified 200 bp from differently treated blood using CCR5 specific primers. (Left Lane: DM2100 marker)

Lane 1: fresh blood
Lane 2: blood +citrate
Lane3: blood +EDTA
Lane 4: blood+EDTA/NaF
Lane 5: blood+Heparin
Lane 6: 1mm2 of dry blood on filter paper

Features
• 5’→3′ DNA polymerase activity
• None detectable 3’→5′ exonuclease (proofreading) activity
• Generates PCR products with 3′-dA overhangs
• Performing PCR directly from blood sample

Applications
• Direct amplification of DNA from blood samples
• High throughput screening without DNA purification

Pack Contents
100ul ExcelTaq Blood Direct DNA Polymerase, (5U/µl, total 500 units)
4x 1ml 5× ExcelTaq Blood Direct PCR Buffer
50ul Positive control primers (10 µM)

Storage Buffer
20 mM Tris(pH 8.0), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, stabilizer, 50% glycerol

Storage
4°C ≥ 6 months
-20°C ≥ 24 months

Recommended PCR Conditions

Blood

3 ul

Forward primer

0.1 ~ 0.5 μM

Reverse primer

0.1 ~ 0.5 μM

10 × Blood Reaction Buffer

5 μl

dNTPs

0.2 mM (each)

Blood Direct Taq DNA Plolymerase

0.25 μl

H2O

to 50 μl

Total volume

50 μl

Recommended PCR Program
94°C                                                       3 min
94°C                           15 sec
50~68°C*                   30 sec
72°C                           1 min/kb
72°C                                                        3 min

*Optimal PCR condition varies according to primers’ thermodynamic properties.

CCR5 Control Primers:
CCR5-F: 5′-CTCCCAGGAATCATCTTTACC-3′
CCR5-R: 5′-TCATTTCGACACCGAAGCAG-3′

Shipping cost
Please note this item is NOT participating the “Free Shipping on $200” rule. A $40 invoice will be sent for a standard 2nd-day shipping. If you order our other Taq PCR related products we only charge shipping cost once. Check our other Taq PCR related products. Buy more save more.

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