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ExcelTaq Klen-Taq DNA Polymerase, TK1000, For Mouse Genotyping

$50.00

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TK1000 ExcelTaq Klen-Taq DNA Polymerase, Great For Mouse Genotyping, Colony Screening. 5 Units/ul, 500 Units/Vial

ExcelTaq Klen-Taq DNA Polymerase (Cat# TK1000) is a specially blended enzyme mix containing KlenTaq-1 DNA polymerase (a 5’-exo-minus, N-terminal deletion of Taq DNA polymerase) and a small amount of a proofreading high fidelity DNA polymerase. This unique blending helps to improve the fidelity, yield and processivity of the resultant PCR process. The Klen-Taq is also highly robust, showing high tolerance of varying concentration of Mg2+; it is highly thermostable and has four times the fidelity compared to regular Taq DNA polymerase. The ExcelTaq Klen-Taq DNA Polymerase is ideal for DNA amplifications 0.5-5 kb in length on mouse, rat, plant genomic DNA, and up to 10 kb on less complex templates, such E coli bacterial genomic DNA, plasmid DNA, Yeast genomic DNA. If you have amplification issues with regular Taq DNA polymerase try our special formula ExcelTaq Klen-Taq DNA Polymerase.

Features

• 5’→3′ DNA polymerase activity
• 3’→5′ exonuclease activity (proofreading)
• Thermo-stable up to 98°C during PCR denaturing step
• 4× fidelity as compared to Taq DNA polymerase
• Robust PCR performance, resistance to variance in PCR condition

Pack Contents

  • 100 ul ExcelTaq Klen-Taq DNA polymerase, 5 units/ ul, total 500 Units
  • 1.2 ml 10x Reaction Buffer

Buffer Recipes

Storage Buffer
40 mM Tris-HCl (pH 7.5), 50 mM KCl, 25 mM (NH4)2SO4, 0.1 mM EDTA, 5.0 mM 2-mercaptoethanol, 50% (v/v) glycerol

10x Reaction Buffer
400 mM Tricine-KOH (pH 9.2 at 25°C), 150 mM KOAc, 35 mM Mg(OAc)2, 750 µg/ ml Bovine Serum Albumin Unit Definition
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.

Storage
-20°C ≥ 24 months

Recommended PCR Conditions

Template

1 ~ 150 ng

Forward primer

0.1 ~ 0.5 μM

Reverse primer

0.1 ~ 0.5 μM

10 × Reaction Buffer

5 μl

dNTPs

0.2 mM (each)

Klen-Taq DNA Plolymerase

0.5 μl

H2O

to 50 μl

Total volume

50 μl

Recommended PCR Program
94°C                                                    2 min
94°C                              15 sec
50~68°C*                      30 sec
68°C                              30 sec/kb
68°C                                                     1 min

*Optimal PCR condition varies according to primers’ thermodynamic properties.

Shipping Method

Our Klen-Taq DNA polyerase is very thermal stable. This item will be shipped at room temperature via Post Office Priority Package which normally delivers within 2 to 3 business day.

Shipping Cost

The shipping cost for each item ordered is $9.50, but will be automatically waived at checkout on orders of $200 and up.

 

 

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