The ExcelTaq SMO-HiFi High Fidelity DNA Polymerase is a new genetic modified, recombinant DNA polymerase with 70 times higher fidelity during amplification than wild type Taq DNA polymerase, as well as very fast elongation rate. Being highly thermostable, the SMO-HiFi high fidelity DNA Polymerase can remain viable even after being subjected to boiling for 2 minutes. The ExcelTaq SMO-HiFi DNA Polymerase is also designed to operate in much lower Mg2+ concentration as compared to other DNA polymerase. Our high fidelity DNA polymerase is great for high GC DNA fragment amplification. SMOBIO HiFi DNA Polymerase is great for gene cloning subcloning applications.
• 5’→3′ DNA polymerase activity
• 3’→5′ exonuclease activity (proofreading)
• High reaction rate (up to 67 base/ second)
• High fidelity, 70 times of Taq polymerase
• Blunt end amplicon
• Thermo-stable-half life is more than 10 hrs at 95°C ion
100 ul ExcelTaq SMO-HiFi DNA Polymerase, 1 U/ul
600 ul 10× Reaction Buffer
500 ul 25mM MgSO4
600 ul dNTPs Mix (2mM each)
600 ul DMSO
50 mM Tris-HCl (pH 8.0), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, stabilizers, and 50% (v/v) glycerol
10x Reaction Buffer
400 mM Tricine-KOH (pH 9.2 at 25°C), 150 mM KOAc, 35 mM Mg(OAc)2, 750 µg/ ml Bovine Serum Albumin Unit Definition
One unit of SMO-HiFi DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-insoluble material in material in 30 min at 74°C.
-20°C ≥ 24 months
Recommended PCR Condition
Template 1 ~ 150 ng
Forward primer 0.1 ~ 0.5 μM
Reverse primer 0.1 ~ 0.5 μM
10 × Reaction Buffer 5 μl
MgSO4 (25 mM) 2 μl
dNTPs (2 mM each) 5 μl
SMO-HiFi DNA Polymerase 1 μl (1 unit)
DMSO 5 μl
H2O 50 μl
Total volume 50 μl
Recommended PCR Program
94°C 2 min
94°C 15 sec
50~68°C* 30 sec
68°C 30 sec/kb
68°C 1 min
*Optimal PCR condition varies according to primers’ thermodynamic properties.
25 ~ 40 cycles
SMO-HiFi DNA Polymerase performs well for high GC content Rhodobactor gDNA templates, with annealing temperature ranging from 59 to 63°C.
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