ExcelTaq SMO-HiFi High Fidelity DNA Polymerase For Gene Cloning, TF1000

TF1000 ExcelTaq SMO-HiFi DNA Polymerase For Gene Cloning. 1 Units/ul, 100 Units/Pack

The ExcelTaq SMO-HiFi High Fidelity DNA Polymerase is a new genetic modified, recombinant DNA polymerase with 70 times higher fidelity during amplification than wild type Taq DNA polymerase, as well as very fast elongation rate. Being highly thermostable, the SMO-HiFi high fidelity DNA Polymerase can remain viable even after being subjected to boiling for 2 minutes. The ExcelTaq SMO-HiFi DNA Polymerase is also designed to operate in much lower Mg2+ concentration as compared to other DNA polymerase. Our high fidelity DNA polymerase is great for high GC DNA fragment amplification. SMOBIO HiFi DNA Polymerase is great for gene cloning subcloning applications.

Features

• 5’→3′ DNA polymerase activity
• 3’→5′ exonuclease activity (proofreading)
• High reaction rate (up to 67 base/ second)
• High fidelity, 70 times of Taq polymerase
• Blunt end amplicon
• Thermo-stable-half life is more than 10 hrs at 95°C ion

Pack Contents

100 ul ExcelTaq SMO-HiFi DNA Polymerase, 1 U/ul
600 ul 10× Reaction Buffer
500 ul 25mM MgSO4
600 ul dNTPs Mix (2mM each)
600 ul DMSO

Buffer Recipes

Storage Buffer
50 mM Tris-HCl (pH 8.0), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, stabilizers, and 50% (v/v) glycerol

10x Reaction Buffer
400 mM Tricine-KOH (pH 9.2 at 25°C), 150 mM KOAc, 35 mM Mg(OAc)2, 750 µg/ ml Bovine Serum Albumin Unit Definition
One unit of SMO-HiFi DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-insoluble material in material in 30 min at 74°C.

Applications

• Generation of blunt end PCR products (proofreading).
• For PCR cloning with GetClone™ PCR cloning vector.

Storage
-20°C ≥ 24 months

Recommended PCR Condition
Template                                     1 ~ 150 ng
Forward primer                       0.1 ~ 0.5 μM
Reverse primer                        0.1 ~ 0.5 μM
10 × Reaction Buffer                             5 μl
MgSO4 (25 mM)                                   2 μl
dNTPs (2 mM each)                               5 μl
SMO-HiFi DNA Polymerase     1 μl (1 unit)
DMSO                                                     5 μl
H2O                                                       50 μl
Total volume                                         50 μl

Recommended PCR Program
94°C                                                    2 min
94°C                          15 sec
50~68°C*                  30 sec
68°C                          30 sec/kb
68°C                                                    1 min
*Optimal PCR condition varies according to primers’ thermodynamic properties.
25 ~ 40 cycles

SMO-HiFi DNA Polymerase performs well for high GC content Rhodobactor gDNA templates, with annealing temperature ranging from 59 to 63°C.

Shipping cost
Please note this item is NOT participating the “Free Shipping on $200” rule. A $40 invoice will be sent for a standard 2nd-day shipping. If you order our other Taq PCR related products we only charge shipping cost once. Check our other Taq PCR related products. Buy more save more.