Our FluoroBand 50 bp Fluorescent DNA Ladder (Cat# DM1160 ) is a ready-to-use DNA ladder, which is pre-mixed with high sensitivity DNA binding fluorescent dye and loading dye for direct loading on gel. This 17 banded 50 bp fluorescent DNA Ladder is composed of 17 individual DNA fragments: 1.5k, 1.2k, 1k, 900, 800, 700, 600, 500, 450, 400, 350, 300, 250, 200, 150, 100, and 50 bp derived from a mixture of PCR products and specifically digested plasmid DNA; these bands can be visualized when illuminated with 470 nm blue light or a UV light. This fluorescent DNA marker contains two enhanced bands (500 bp and 200 bp) for easy referencing. In addition, the low range Orange G tracking dye which mimics the migration of a 50 bp dsDNA during electrophoresis is also added for real time monitoring. Real time observation of the electrophoresis is also possible if compatible light source is fitted to the electrophoresis tank.
Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.
50 ~ 1,500 bp
54 µg/ 500 µl
Recommended loading volume
25 ~ 5 µl/ well. 2.5ul for small well and 5ul for large well agarose gels
Protected from light
Room temperature ≥ 6 months
4°C ≥ 12 months
-20°C ≥ 36 months
The shipping cost is $9.50 for each order placed, but will be automatically waived at checkout on orders of $200 and up. If your total order is less than $200, free shipping cost for extra or other DNA ladders ordered in the same order.