The SMOBIO FluoroBand XL Broad Range 25kb Fluorescent DNA Ladder (Cat# DM4160) is a ready-to-use DNA ladder, which is pre-mixed with high sensitivity DNA binding fluorescent dye and loading dye for direct loading on gel. SMOBIO broad range fluorescent DNA Ladder DM4160 is composed of 14 individual DNA fragments: 25k, 10k, 8k, 6k, 5k, 4k, 3k, 2.5k, 2k, 1.5k, 1k, 750, 500, and 250 base pairs derived from a mixture of PCR products and specifically digested plasmid DNA; these bands can be visualized when illuminated with 470 nm blue light or UV light. This 25Kb broad range fluorescent DNA marker contains two enhanced bands (3 kb and 1 kb) for easy referencing. In addition, two tracking dyes, Xylene cyanol FF and Bromophenol blue which mimic the migration of 4,000 bp and 500 bp dsDNA during electrophoresis are also added for real time monitoring. Real time observation of the electrophoresis is also possible if compatible light source is fitted to the electrophoresis tank.
Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.
100 ~ 10,000 bp
51.6 µg/ 500 µl
Recommended loading volume
25 ~ 5 µl/ well. 2.5ul for small well and 5ul for large well agarose gels
Protected from light
Room temperature ≥ 6 months
4°C ≥ 12 months
-20°C ≥ 36 months
The shipping cost is $9.50 for each order placed, but will be automatically waived at checkout on orders of $200 and up. If your total order is less than $200, free shipping cost for extra or other DNA ladders ordered in the same order.