$200/1000 grams high pure quick dissolve super clear agar. Free ground shipping on orders $200 and up. Agar, also called Agar-agar, is a gelatin-like product made primarily from the algae Gelidium and Gracilaria (red seaweeds). Best known as a solidifying component of bacteriological culture media. The high pure agar is produced by South Korea manufacturer through a greener way which eliminates organic chemicals treatment. Our high pure agar maximizes the growth of your bacteria and gives clear better vision for microscopy. Most of customers found 12g can generate 1 liters pretty solid agar plates.
Features & Advantages
Gel Point (1.5%): 32-38°C; Gel Strength (1.5%): 550-950 g/cm2;
Heavy Metals: ≤0.004%; Loss on Drying: ≤10.0%;
Melting Point: 80-90°C; Pass Thru 60 Mesh: ≥90(%);
pH (1.5%): 6.0-7.6; Turbidity (Nephelos): ≤40;
Solubility (Boiling Water): Clear; Storage: dry @ room temperature
For 1 liter (1000ml):
Weight 10g Tryptone (or peptone); 5g Yeast extract; 5g NaCl. Add distilled water to 900ml in a 2 liter flask, completely dissolve the mixture. Adjust pH to 7.0 (normally 200ul 10N NaOH). Add 12-15 grams of our high pure agar into the solution, mix well, and add H2O to 1000ml. The agar will not dissolve until autoclaved. Autoclave for 30~45 minutes at 121°C using liquid cycle. When the agar has been autoclaved, allow it to cool around 70°C. Gently shake the flask to mix agar. Let it continue to cool down until the flask can be held with bare hands. Ad 1ml antibiotics or any compound you would like to add. Mix thoroughly and let the medium to sit for 5min. If you pour the medium immediately after mix, there will be many small bubbles in your plates after incubation at 37°C. After 5min, carefully pour out into sterile plastic petri dishes. You can make 40 to 50 10cm petri dishes at 20-25ml/10cm plate. Allow to set, then store wrapped in plastic in the cold room. Remember to label them with date and correct antibiotics. Shelf time: kanamycin plates are good for around 1 year, and Ampicilin plate are good for around 6 months. If you don’t use so much we suggest you scale down to make less plates.
100 grams/Bag, 1000 grams/2x Bottles, 500 grams/Bottle