$140/kit of 50 preps for microRNA total RNA Miniprep Kit with patented O-ring columns. Get pure, washing buffer free total mRNA, microRNAs, and reliable, reproducible quantitative real time PCR results. Free ground shipping on orders $200 and up.
DNALand Scientific provides microRNA total RNA Miniprep Kit, A simple spin-column based method can simultaneously isolate large RNAs, small RNAs, including microRNAs, and viral RNAs without the time-consuming procedure of phenol/chloroform extraction and ethanol precipitation.
DNALand Scientific microRNA plus normal message RNA (miTotal™ RNA) Extraction Miniprep Kit, microRNA total RNA Miniprep Kit, provides a fast method to purify total RNA from various samples such as cells from culture, tissues, whole blood, plasma, serum, biological fluids containing RNA virus, etc.
Compared with regular total RNA isolation kit our microRNA total RNA Miniprep Kit allows researchers to isolate microRNA, small RNA and large message RNA (mRNA) simultaneously. This kit is great for research who want to check the relationship between one specific microRNA and one or more normal mRNA.
Our revolutionary O-ring design of the purification column eliminates solvent resides from your RNA samples, which generates more consistent reverse transcription and more accurate and reliable qPCR results.
Are you still using RNA purification kit (RNeasy Mini Kit) with traditional isolation columns for your RNA isolation? Test this before you add RNase-free water to the column to elute your RNAs, move a small tip connected to vacuum on the O-ring of inside of the column to see what you will get. Yes, washing buffer! even you centrifuge for over 30min, the washing buffer is still on the top of the O-ring, which leads to the low A260/230 ratio of your samples. Especially when your RNA concentration is low.
Our competitors never find the reason why their columns give low A260/230 ratio, or never think about how to resolve the problem if they have found the problem. Switch to our less expensive kit, enjoy the patented design and get high A260/230 ratio, and more accurate qRT-PCR results.
If both mRNA and microRNA will be studied, this is the right kit for you. MicroRNA (>17nt) can also be enriched and purified efficiently.
Features and benefits
>Cells from culture, tissues, whole blood, plasma, serum, biological fluids containing RNA virus.
>Sample size:1×104 – 5×106 animal cells, up to 0.1 g tissue and up to 300 µl biological fluids
>Purification process requires only 20-30 minutes
>Spin column fit by centrifugation
>No toxic reagent (no phenol/chloroform, no CsCl gradients, no LiCl precipitation)
>Ready-to-use high-quality RNA (Purity A260 /A280 >1.9) from small to large amounts of starting material
>Binding capacity up to 50ug
>Generate washing buffer free RNA samples
>More accurate and consistent quantitative real-time PCR (qRT-PCR) result.
RNA purified by our microRNA total RNA Miniprep Kit has A260/A280 ratio of 1.9~2.1, and is ideal for all applications including:
>Northern, dot and slot blotting
>RT-PCR / Quantitative real-time PCR
>Poly A+ RNA selection
>cDNA Synthesis / Primer extension
>In vitro translation
>microRNA expression assay
The Facts of Wash Buffer Containing RNA Samples
Lead to low A260/230. It will be more obvious when you have a low RNA concentration. After you normalize your RNA samples to the same concentration, the wash buffer in each concentration-normalized samples are not the same. The original low concentrated RNA samples will have more wash buffer in the concentration-normalized samples because you will add more wash buffer-containing-samples to get the same quantity. When you do reverse transcription, reverse transcriptase activity will be inhibited. The more wash buffer in your samples the more inhibition of reverse transcriptase activities. Thus, there will be less cDNA in the original low RNA samples although you put same amount RNA in the reverse transcriptase reaction.
The final consequences will be: when you do quantitative real-time PCR you will get non-accurated results; when you clone genes, especially for those low expression genes, you will get very low PCR products or couldn’t get any PCR products.
The shipping cost for each case ordered is $9.50, but will be automatically waived at checkout on orders of $200 and more.