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Q-HiFi DNA Polymerase, Quick High Fidelity DNA Polymerase, TF2000

$90.00 $70.00

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Quick High Fidelity DNA Polymerase, Q-HiFi DNA Polymerase (Cat# TF2000). 1U/ul, 100U/Pack

Our quick high fidelity DNA polymerase, Q-HiFi™ DNA Polymerase (TF2000) is a unique blended enzyme mix containing a high fidelity DNA polymerase and an elongation enhancer, thus being capable to perform quick and accurate PCR. The extension rate of Q-HiFi™ DNA Polymerase (7 sec/kb) is > 4-fold quicker than that of pfu DNA polymerase (30 sec/kb). The fidelity of Q-HiFi™ DNA Polymerase is 50 times higher than that of Taq DNA polymerase. The optimized 5X Q-HiFi™ Buffer includes special enhancers that contribute to suppress non-specific amplification as well as plateau effect produced by conventional PCR. Therefore, fast fidelity Q-HiFi™ DNA Polymerase exhibits great elongation capability (up to 40 kb from lambda DNA).

Features
•5’→3’ DNA polymerase activity
•3’→5’ exonuclease (proofreading) activity
•High reaction rate: 7 seconds/kb
•High fidelity: 50 times higher than Taq polymerase
•Generates blunt end amplicons
•Vast elongation capability (up to 40 kb)
•Thermo-stable for more than 10 hrs at 95°C

SMOBIO-Q-HiFi-DNA-polymerase-TF200

Fig. 1. Q-HiFiTM DNA Polymerase’s high processability enables reliable amplification of λDNA up to 40 kb in length (M: DM3100 ).

TF2000-Q-HiFi-DNA-Polymerase

Fig. 2. Q-HiFiTM DNA Polymerase can amplify PCR products from as little as 1 fg of template DNA (M: DM2000).

Applications
Long range PCR amplification for next-generation DNA sequencing
Generates blunt end amplicons for cloning with GetClone™ PCR cloning vector

Pack Contents

100 ul Q-HiFi High Fidelity DNA Polymerase, 1U/ul
1200 ul 5× Q-HiFi Reaction Buffer
600 ul dNTPs Mix (2mM each)

Storage Buffer
50 mM Tris-HCl (pH 8.0), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, stabilizers, and 50% (v/ v) glycerol

Unit Definition
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.

Storage

-20°C ≥ 24 months

Recommended PCR Conditions

Template

1 ~ 150 ng

Forward primer

0.1 ~ 0.5 μM

Reverse primer

0.1 ~ 0.5 μM

5 × Reaction Buffer

10 μl

dNTPs

0.2 mM (each)

Q-HiFi High Fidelity DNA Plolymerase

1 μl

H2O

to 50 μl

Total volume

50 μl

Recommended PCR Program For ≦10 kb products:
98°C                                                              2 min
98°C                          15 sec
50~68°C*                  30 sec
68°C                          10 ~30 sec/kb
68°C                                                               1 min

*Optimal PCR condition varies according to primers’ thermodynamic properties.

Recommended PCR Program For ≧10 kb products:

98°C                          10 sec
68°C                          10 ~30 sec/kb

Shipping cost
Please note this item is NOT participating the “Free Shipping on $200” rule. A $40 invoice will be sent for a standard 2nd-day shipping. If you order our other Taq PCR related products we only charge shipping cost once. Check our other Taq PCR related products. Buy more save more.

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