$165/kit of 50 single cell total RNA isolation kit, which isolate microRNA, total mRNA simultaneously from as little as one single cells. Free ground shipping on orders $200 and up.
DNALand Scientific Single cell miTotalTM RNA Extraction Miniprep kit provides a fast method to purify total RNA of a single cell from various samples such as cells from culture or blood buffy coat.
A simple spin-column based method can isolate large RNAs, siRNAs and microRNAs without the time-consuming procedure of phenol/chloroform extraction and ethanol precipitation, the highly purified total RNA can be used for quantitative detection of RNA level using quantitative method, such as qPCR, digital PCR or microarray.
This kit also uses our patented O-ring Column Design to eliminate wash buffer residues (traditional kit has this problem) from eluted RNA samples. The wash buffer-free RNA samples will generate best results for your down stream applications.
* Digital PCR
* cDNA synthesis
The Facts of Wash Buffer Containing RNA Samples
Lead to low A260/230. It will be more obvious when you have a low RNA concentration. After you normalize your RNA samples to the same concentration, the wash buffer in each concentration-normalized samples are not the same. The orignal low concentrated RNA samples will have more wash buffer in the concentration-normalized samples because you will add more wash buffer-containing-samples to get the same quantity. When you do reverse transcription, reverse transcriptase activity will be inhibitted. The more wash buffer in your samples the more inhibition of reverse transcriptase activities. Thus, there will be less cDNA in the orginal low RNA samples although you put same amount RNA in the reverse transcriptase reaction.
The final consequences will be: when you do quantitative real-time PCR you will get non-accurated results; when you clone genes, especially for those low expression genes, you will get very low PCR products or couldn’t get any PCR products.
The shipping cost for each case ordered is $9.50, but will be automatically waived at checkout on orders of $200 and more.