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SYBR Green Real-Time PCR Master Mix, Quantitative PCR Master Mix

$80.00 $60.00

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SYBR Green Real-Time PCR Master Mix, SYBR Green Quantitative PCR Master Mix. 200 Reactions

SMOBIO SYBR Green Real-Time PCR Master Mix, SYBR ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX, TQ1110) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers and template. The 2x master mix features high sensitivity (Fig. 1) and high signal intensity when compared with Brand A (Fig. 2). The ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX) contains hot-start Taq polymerase in an optimized buffer with dsDNA specific SYBR green fluorescent dye. This master mix allows for sensitive and precise amplification, real-time tracking of the amplification process, and simultaneous quantification for targeted DNA molecules. The master mix includes ROX reference dye for normalization of each qPCR assay. With inert smart blue contrast dye, our  SYBR Green Real-Time PCR Master Mix — ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process. Producing a reliable and reproducible gene expression profile for your research.

Features

•High sensitivity and signal intensity
•With ROX reference dye
•Smart blue contrast dye as a visual aid for reaction setup
•Better compatibility for reverse transcription

Application
Quantitative real-time PCR
Quantitative two-step real-time PCR

Pack Contents

2x SYBR Green Real-Time PCR Master Mix:   2x 1ml

sybr-green-qPCR-Master-Mix-amplification-plot

Fig. 1. The amplification plot of real-time PCR with cDNA templates ranging from 1.49 fg to 25 ng in quantity, analyzed by using TQ1110 ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX) for qPCR amplification.

comparison-qPCR-amplification-curve

Fig. 2. SMOBIO’s TQ1110 ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX) shows better fluorescent signal as compared to a similar product (Brand A).

Guide for use

For best results of Q-PCR by using 2X Q-PCR Master Mix (SYBR, ROX), the following procedures and components listed in Tables 1 to 3 are strongly suggested.

Table 1. Recommended reaction mixture set up for Q-PCR

Reaction Components

Volume

Final Concentration

Template

2ul

CDNA: 149 fg ~ 25ng

gDNA: 80 pg ~ 25ng

Plasmid: 102 ~ 108 molecules

Forward Primer

varied

50 ~ 400 nM

Reverse Primer

varied

50 ~ 400 nM

2x qPCR Master Mix (SYBR, ROX)

10ul

1x

ddH2O

Up to 20ul

Total Volume

20ul

* The PCR primer concentration for an optimal Q-PCR reaction may vary according to the primer’s properties and template conditions.

Table 2. Recommended real-time PCR program for: Two-step cycle

Steps

Temp

Time

Cycles

Template Denaturation and Enzyme Activation

95℃

10 min

1

Denaturation

95℃

15 sec

40

Annealing/Extension

60℃

60 sec

Table 3. Recommended real-time PCR program for: Three-step cycle

Steps

Temp

Time

Cycles

Template Denaturation and Enzyme Activation

95℃

10 min

1

Denaturation

95℃

15 sec

40

Annealing

60℃

30 sec

Extension

72℃

30 sec

Shipping cost
Please note this item is NOT participating the “Free Shipping on $200” rule. A $40 invoice will be sent for a standard 2nd-day shipping. If you order our other Taq PCR related products we only charge shipping cost once. Check our other Taq PCR related products. Buy more save more.

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