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Total RNA Isolation Miniprep Kit, RNA Isolation Extraction From Tissue Cell Bacteria Yeast

$135.00

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SKU: GR1001 Categories: , , Tag:

Total RNA Isolation Miniprep Kit,  RNA Extraction Mini Prep Kit, 50/kit, $130/kit

$135/kit of 50 total RNA isolation miniprep kit with O-ring columns which eliminate washing resides in the eluted total RNAs. Get more reliable qPCR results and more cDNAs. Free ground shipping on orders $200 and up.

DNALand Scientific Total RNA Isolation Minprep Kit, Extraction MiniPrep Kit (50 preps) provides an economical method to purify total RNA from various samples such as mammalian cultured cells, animal tissues, bacteria and yeast.

A simple silica-gel-membrane spin-column method can isolate total RNA without phenol/chloroform extraction and ethanol precipitation. This kit contains our patented O-ring design which eliminates washing buffer from eluted RNA samples. With our kit you can get best A260/280 ratio of your samples, no reverse transcriptase inhibition, more precise and consistant quantitative real time PCR results.

Our total RNA Isolation Miniprep kit also uses our patented O-ring Column Design to eliminate wash buffer residues (traditional kit has this problem) from eluted RNA samples. The wash buffer-free RNA samples will generate best results for your down stream applications.

Features & Advantages
   >Purification process requires only 20~30 minutes

   >Spin column fit by centrifugation
   >No toxic reagent (no phenol/chloroform, no CsCl gradients, no LiCl precipitation)
   >Ready-to-use high-quality RNA (Purity A260 /A280 >1.9) from small to large amounts of starting material

The yields of Total RNA Extraction System from various starting materials

Sample

Sample Size

Elution Volume

DNA Yield

Animal Tissue

10 ~ 30 mg

30 ~ 50 ul

Up to 30 ug

Culture Cells

Up to 1 x 107 Cells

30 ~ 50 ul

Up to 45 ug

Bacteria

Up to 1 x 109 Cells

30 ~ 50 ul

Up to 65 ug

Yeast

Up to 5 x 107 Cells

30 ~ 50 ul

Up to 65 ug

Downstream Applications

RNA purified by DNALand Scientific Total RNA Extraction MiniPrep Kit has A260/A280 ratio of 1.9~2.1, and is ideal for all applications including: 

  • Northern, dot and slot blotting
  • RT-PCR / Quantitative real-time PCR
  • Poly A+ RNA selection
  • cDNA Synthesis / Primer extension
  • Array analysis
  • In vitro translation

The Facts of Wash Buffer Containing RNA Samples
Lead to low A260/230. It will be more obvious when you have a low RNA concentration. After you normalize your RNA samples to the same concentration, the wash buffer in each concentration-normalized samples are not the same. The orignal low concentrated RNA samples will have more wash buffer in the concentration-normalized samples because you will add more wash buffer-containing-samples to get the same quantity. When you do reverse transcription, reverse transcriptase activity will be inhibitted. The more wash buffer in your samples the more inhibition of reverse transcriptase activities. Thus, there will be less cDNA in the orginal low RNA samples although you put same amount RNA in the reverse transcriptase reaction.

The final consequences will be: when you do quantitative real-time PCR you will get non-accurated results; when you clone genes, especially for those low expression genes, you will get very low PCR products or couldn’t get any PCR products.  

Please check out our low-cost Min Plus plasmid DNA isolation kit and Gel extraction PCR clean-up 2-in-1 kit with patented O-ring design for your daily molecular works.

Shipping cost
The shipping cost for each item ordered is $9.50, but will be automatically waived at checkout on orders of $200 and up.

 

 

 

 

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